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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in lots of tumors, which results in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a critical job in advertising and marketing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two more splice variants had been reported but thorough nucleotide sequence facts just isn't nonetheless offered.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide usually includes the epitope recognized through the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By evaluating the staining through the blocked antibody as opposed to the antibody alone, one can see which staining is particular.
Goal Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins show distinct expression and degradation styles which add for the temporal coordination of each mitotic occasion. Cyclin E sorts a fancy with and functions as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells development as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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